Overview

In-Fusion Snap Assembly is a simple yet elegant cloning technology that has been optimized to give you exceptionally high accuracy, guaranteeing cloning success and saving you time and hassle.

• Subcloning is unnecessary: clone any insert directly into your final vector, regardless of your cloning locus

• Highly efficient: over 95% efficiency demonstrated with a broad range of fragment sizes, from 0.5 kb to 34 kb*

• Seamless construction: final constructs have no extra base pairs left over (as is often the case with restriction digest or TA cloning)

• Flexible experimental design: clone single or multiple DNA fragments simultaneously, with just a single reaction

• Site-directed mutagenesis: click here to learn how this streamlined technology can be applied to insertions, deletions, or substitutions

Product Description

In-Fusion Snap Assembly products enable directional, seamless cloning of any PCR fragment—or multiple fragments—into any linearized vector in a single 15-minute reaction. No additional treatment of the PCR fragment (such as restriction digestion, ligation, phosphorylation, or blunt-end polishing) is required. The efficiency of In-Fusion Snap Assembly is over 95%, providing confidence in your clones and enabling you to scale up for high-throughput workflows.

Substance

In-Fusion Snap Assembly Master Mix※ (Product Codes 638947, 638948, 638949, 638943, 638944) 5X In-Fusion Snap Assembly Master Mix pUC19 Control Vector, linearized 2 kb Control Insert

Preservation

−20°C

Visit Takara Bio website : https://www.takarabio.com/products/cloning/in-fusion-seamless-cloning/in-fusion-snap-assembly?catalog=638947